============ Introduction ============ CellProfiler Analyst (CPA) provides tools for classifying biological images and exploring and visualizing multidimensional data (particularly from high-throughput experiments) that has been extracted from companion image analysis software CellProfiler. CellProfiler Analyst’s primary tools: * **Image Gallery** displays (full and cell) images with a variety of filter options and can be used interactively with other tools. * **Classifier** enables cell and field-of-view-level classification of multiple phenotypes using popular supervised machine learning models. * **Plate Viewer** displays data according to the spatial layout of the experiment, such as a multi-well plate or microarray. * **Scatter Plot**, **Histogram**, and **Density Plot** display numerical data. * **Table Viewer** displays numerical and text data in a spreadsheet format, where data points can be clicked to display images. * **Normalization Tool** creates a new datatable with normalized and feature-selected columns. The CellProfiler project is based at the Broad Institute Imaging Platform. It was started by `Anne E. Carpenter `_ and `Thouis (Ray) Jones `_ in the laboratories of David M. Sabatini and Polina Golland at the Whitehead Institute for Biomedical Research and MIT's CSAIL. `David Dao `_ currently leads the development of CellProfiler Analyst. CellProfiler Analyst is tested on MacOS 10.10 and 10.11, Windows 7 and 10, and to a lesser extent Linux. Running the software from Linux currently requires use of the source code, which is beyond the scope of this document. CellProfiler Analyst is distributed under the BSD-3 Clause (see LICENSE.txt).